​Bituminous carbonate rocks of the Upper Cretaceous Shu’ayb Formation from the Ajloun outcrop in Northern Jordan were geochemically and petrologically analyzed. This study integrates kerogen microscopy with a finding from geochemical results (i.e., biomarker, stable carbon isotope and major elemental compositions) to understand the organic matter inputs and to reveal the dispositional setting and its effect on the occurrences of organic matter. The Shu’ayb bituminous carbonate rocks have high total organic carbon (TOC) and sulfur values, with average of 12.3 and 4.59 wt. %, respectively, indicating redox conditions during their precipitation. The high abundant of alginite (i.e., lamalginite) in the Shu’ayb bituminous carbonate sediments is a further evidence for a redox conditions. The finding of mainly marine-derived organic matter is also demonstrated by the biomarker distributions and carbon isotope. The biomarkers are represented by narrow Pr/Ph ratios of up to 0.97, abundant of tricyclic terpanes and high C27 regular sterane, indicating that the organic matter (OM) was primarily derived from mainly phytoplankton algae, along with small amounts of land plant-derived material and were accumulated under reducing conditions. The studied Shu’ayb bituminous carbonate facies is composed of mainly calcium (CaO= average 45.10 wt%), and significant amounts of silicon (Si2O3= avg. 9.35 wt%), aluminum (Al2O3= avg. 6.91 wt%), and (P2O3= avg. 1.47 wt%), with low amounts of iron (Fe2O3) and titanium (TiO2) of less than 1 wt%, indicating that the detrital influx was low in an open water depth system with higher primary bioproductivity. The geochemical proxy suggests that the Shu’ayb bituminous carbonate facies was established in a saline water environment, with Ca/Ca+Fe and S/TOC values of more than 0.9 and 0.50, respectively, which could attributed to increasing in reducing conditions of the water column. The chemical​ index of alteration values of more than 0.8 also indicate that the Shu’ayb bituminous carbonate facies took place during the warm and humid climatic conditions, thereby resulting in an intensity of subaerial weathering.​