Fusarium crown rot (FCR) is caused by many Fusarium species, primarily F. culmorum (W. G. Sm.) Sacc., F. pseudograminearum (O’Donnell & T. Aoki), and F. graminearum Schwabe (Paulitz et al. 2002). It is a global wheat disease causing large yield losses. In the Middle East, it was reported in Iraq (Matny et al. 2019; Motallebi et al. 2015) and Syria (Motallebi et al. 2015). In Jordan, Fusarium on wheat was only noted once, in a checklist (Mamluk et al. 1984) without identifying the species or pathogenicity. FCR symptoms were observed in 2016 to 2022 (Alananbeh et al. 2018) across Jordan in annual wheat disease surveys. Disease severity was higher in dry seasons, such as in 2017 and 2021. There were very severe symptoms at four University of Jordan experimental wheat plots in 2016 to 2022, where 40 symptomatic plants were randomly collected. Roots and stems were cut into small sections, disinfected in 0.5% NaOCl for 5 min and 70% ethanol for 1 min, rinsed in sterile distilled water three times, dried under laminar flow, plated on PDA, and incubated for 10 to 14 days at 25°C. Cultures were purified by hyphal tipping. At least one pure isolate with typical F. culmorum morphology was recovered from each plant. Pure cultures grew rapidly on PDA with fluffy floccose aerial mycelium and dark red to reddish brown pigment diffused in the agar. Isolates produced monophialidic conidiogenous cells. Macroconidia were slightly curved, with pointed apical and foot cells, 3 to 5 septate, on average 28.5 to 46.5 × 4.5 to 7.0 µm, indicating they were Fusarium spp. Chlamydospores were intercalary in hyphae and microconidia were absent. Two representative isolates (Iso-1 and Iso-2) identified putatively as F. culmorum based on morphology, were sent to Macrogen, South Korea, to Sanger sequence a portion of the translation elongation factor 1-a gene using the EF1/EF2 primers (Geiser et al. 2004). Raw sequences were used to create consensus sequences in BioEdit. BLASTn queries of the consensus sequences of the two isolates found they were 99.67 and 100% identical to MW233082.1, a TEF1-a sequence of the F. culmorum ex-epitype (NRRL 25475; Crous et al. 2021). The sequences were accessioned in GenBank (OQ785278 and OQ785279). Thus, Iso-1 and Iso-2 were identified as F. culmorum. Pathogenicity was tested on two wheat cultivars, Hourani and Norsi, both in vitro on seeds and on seedlings. A 4 × 104 macroconidia suspension was prepared from a 10-day-old culture on PDA at 28°C. Seeds of both cultivars were surface sterilized in 1% (v/v) bleach and rinsed in sterile distilled water three times. For the seed test, seeds were soaked in the suspension for 15 min and dried with filter paper. Seeds were plated onto sterile paper towels in sterile plastic boxes and put in a growth chamber. Three replicates (10 seeds/replicate) were used. Controls were treated with sterile distilled water. Percent germination, coleoptile length, radicle length, longest seminal root length, and number of seminal roots were measured after 5 days. For the seedling test, seeds were planted in trays with sterilized 1:1:1 peat moss/sand/soil. A 5 ml suspension was drenched following seedling emergence. Ten replicates (1 seed/replicate) were used. Plants were watered as necessary. Controls were drenched with sterile distilled water. Symptoms were rated by the disease severity index (Mitter et al. 2006) after 35 days. The seed test showed reduced germination and other measurements in the presence of F. culmorum. Seedling height, length of discoloration, disease score, disease severity index, and percent germination were reduced in F. culmorum treated seedlings. Norsi was more susceptible to FCR than Hourani in both tests. F. culmorum was reisolated from roots of inoculated plants of both cultivars. This is the first report of F. culmorum on wheat in Jordan. It can cause large losses and research is ongoing to survey FCR-associated Fusarium spp. in Jordan, their genetic diversity, and QTL mapping for resistance genes in wheat landraces.