​Immunolocalization of tomato brown rugose fruit virus (ToBRFV) on tissue printing of different parts of contaminated (A-C) and healthy (D-F) tomato seeds. Half seeds were pressed with the coat face down (A, D), with the coat face up (B, E), with the dissected embryo only (C, F). c: cotyledons, e: embryo, es: endosperm, h: hair, r: radicle, t: testa. Arrows indicate colored precipitate signal, specific for virion detection.