​A novel carlavirus has been discovered in Carica papaya by high-throughput sequencing of a sample collected during field surveys in 2022 from Jordan Valley, Jordan. The complete genome sequence of 9169 nt was determined by RACE and verified by Sanger sequencing, and exhibited the typical organization of members of the genus Carlavirus, including a predicted ORF6. Alignment of coat protein and polymerase aa sequences revealed closest identity (~94% and ~69%, respectively) to a cucumber vein-clearing virus (CVCV) isolate. According to the ICTV demarcation criteria based on sequence alignment, the virus is a tentative new member of the genus Carlavirus, and the name “papaya virus B” is proposed. Following field surveys, the virus was detected in 20 out of 38 papaya samples with clear virus-like symptoms collected from 5 fields in Jordan, showing occurrence of infections with the novel carlavirus in different cultivated areas across Jordan Valley. ​