Practical blood flow restriction (PBFR) is an innovative technique that reduces the venous return through the application of a pressure band calibrated using a perceived pressure scale. Owing the scarcity of studies investigating the acute effects of PBFR in conjunction with low-load resistance exercises versus traditional high-intensity exercises on growth hormone (GH) and blood lactate (BL) levels following lower extremity training in collegiate athletes, this study sought to bridge this gap. The research aimed to explore the acute impact of a single session low-load resistance exercises with PBFR, comparing it with high-load resistance exercises without PBFR, specifically focusing on GH and BL levels. A convenience sample of collegiate athletes was divided into an experimental group engaging in low-resistance exercises with PBFR and a control group performing high-resistance exercises without PBFR. GH and BL levels were assessed at three intervals: pre-training, immediately post-training, and 15-min post-training for both groups. There was no significant differences between groups in age, BMI, and the number of training hours per week. Analysis of variance (ANOVA) was employed to elucidate the main and interaction effects between the groups. Notably, a significant main effect of time on GH and BL levels was observed in both groups. While no significant differences emerged in GH secretion between the groups, a notable variance was found in BL secretion levels. This suggests that both low-resistance exercises with PBFR and high-resistance exercises without PBFR can elicit a similar effect on GH secretion. In contrast, the method of high-resistance exercises without PBFR may induce a more pronounced effect on BL secretion. In conclusion, this research demonstrates that PBFR implementation offers athletes dual benefits: it equivalence with low-load exercises, enabling benefits while using lighter training loads, and mitigating BL accumulation for sustained training.​​