​In sports, session rating of perceived exertion (sRPE) is used to reflect internal training load (TL). TL reflects the body physiological strains during exercise sessions. The extent to which​ exercise intensity and duration affect sRPE during successive football training sessions is unclear. The study investigated the impact of exercise intensity and duration on the session rating of perceived exertion (sRPE) in relation to physiological variables during football training sessions. A sample of 47 youth male football players underwent two high-intensity exercise trainings with a 15-minute break in between. The first training consisted of three 15-minute "walk-sprint-jog" sessions, followed by three minutes of recovery. The second training continued until exhaustion. The levels of sRPE, physiological data, blood and urine analysis were assessed pre-exercise, after each session, and after exhaustion. Results showed a progressively significant increase in sRPE, physiological, blood and urine parameters from the first session until exhaustion. The impact of cumulative duration on the holistic perception of workload showed a linear increment during consecutive exercise sessions. The study concludes that sRPE demonstrates sensitivity to the accumulation of perceived fatigue resulting from exercise duration during football training sessions, even with consistently maintained exercise intensity.​