​Dr.Abeer Al-Qatati spoke at the International Conference of Medical Laboratory and Health Sciences Research at Zarqa University-Jordan. Using ELISA Analysis to Determine Protein Expression Patterns in HepG2 Cells in Response to Insulin, Glucose, and the Anti-Diabetic Bis (Maltolato)Oxovanadium ( IV)

Type 2 diabetes, the most common type of diabetes mellitus is caused by insulin insensitivity. Many researches have been accomplished to find orally active therapeutic compounds that could replace insulin injections. In this study, we aimed to examine the expression levels of several key proteins involved in insulin receptors and AMPK signaling pathways in response to treatments with insulin, glucose and/or BMOV by using ELISA. We found that when glucose was added, treatment of HepG₂ cells with BMOV for 24 hours was associated with a higher GLUT4 concentration in cell lysate, AMPKα_1, and AMPKα₂ in the cell supernatant. In addition, treatment with BMOV for 1 hour was associated with a higher insulin receptor concentration in HepG2 cell lysate. For cell-based ELISA, the expression level of AMPKγ was higher in the presence of BMOV for 1 hour with glucose. Treatment with insulin for 1 hour and BMOV for 24 hours in the presence of glucose caused a high expression of AMPKβ1 isoform. Also, Western blot analysis revealed that the expression of Glut4 was highest under BMOV treatment for 1 hour and insulin treatment for 1 hour was associated with a high expression of AMPKα₁ that was comparable to the “no treatment" control. In conclusion, BMOV could target the AMPK system, a major tool in treating obesity, diabetes, and metabolic syndrome.​

Abeer.pptx