Type 2 diabetes mellitus (T2DM) is a chronic and complex disease associated with several complications. Among them, diabetic foot ulcer (DFU) stands out as a critical concern due to their associations with infections, poor and delayed wound healing, and a high risk of lower limb amputation. DFU has a complicated pathophysiology and includes poorly understood molecular and metabolic changes. However, there is a need for potential biomarkers for early detection, prognosis, and personalized therapeutic interventions. Metabolomics has emerged as a powerful tool in biomedical research, offering a comprehensive snapshot of the small molecules present in biological samples. This approach holds immense promise for unraveling the intricate metabolic signatures associated with diseases. This project aims to investigate the metabolomic fingerprint of diabetic foot ulcer patients using an untargeted liquid chromatography-mass spectrometry (LC-MS) analysis to profile the dynamic and local changes in endogenous metabolites. Blood and tissue samples will be collected from diabetic patients with DFU, diabetics without DFU, and matched nondiabetic controls.
The Project is Funded by The Deanship of Scientific Research/The University of Jordan (JD 20000)